Purified anti-human CD314 (NKG2D) Antibody

Pricing & Availability
Clone
1D11 (See other available formats)
Regulatory Status
RUO
Other Names
NKG2D
Isotype
Mouse IgG1, κ
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Product Citations
publications
1D11_Pure_020708
Human peripheral blood lymphocytes were stained with purified CD314 (clone 1D11) (filled histogram), or purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
  • 1D11_Pure_020708
    Human peripheral blood lymphocytes were stained with purified CD314 (clone 1D11) (filled histogram), or purified mouse IgG1, κ (open histogram), followed by anti-mouse IgG FITC.
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320802 100 µg $100.00
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Description

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8+ T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu J, et al. 1999. Science 285:730.
  2. Wu J, et al. 2000. J. Exp. Med. 192:1059.
  3. Groh V, et al. 2001. Nature Immunol. 2:255.
  4. Wu J, et al. 2002. J. Immunol. 169:1236.
  5. Roberts A, et al. 2001. J. Immunol. 167:5527.
  6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
  7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
  8. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  9. Watanbe M, et al. 2014. Int Immunol. PubMed
Product Citations
  1. Chen HC, et al. 2017. Immunol Cell Biol. 95:620. PubMed
  2. Nakajima NI, et al. 2017. Oncol Rep. 38:693. PubMed
  3. Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
  4. Kraetzel K, et al. 2008. Eur Respir J. 32:563. PubMed
  5. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
RRID
AB_492956 (BioLegend Cat. No. 320802)

Antigen Details

Structure
C-type lectin
Distribution

NK cells, γ/δ T cells, CD8+ T cells

Function
Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor
MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type
NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.

Gene ID
22914 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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