Purified anti-human CD309 (VEGFR2) Antibody

Pricing & Availability
Clone
A16085I (See other available formats)
Regulatory Status
RUO
Other Names
VEGFR2, KDR
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16085I_PURE_CD309_Antibody_1_IHC-P_050817
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD309/VEGFR2 (clone A16085I) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A16085I_PURE_CD309_Antibody_1_IHC-P_050817
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD309/VEGFR2 (clone A16085I) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A16085I_PURE_CD309_Antibody_2_IHC-P_050817
    Human paraffin-embedded breast cancer section slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD309/VEGFR2 (clone A16085I) purified at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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374002 100 µg $195.00
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Description

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VEGFR2-Fc Chimera
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687130 (BioLegend Cat. No. 374002)

Antigen Details

Structure
Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
Distribution

Endothelial cells, embryonic tissues

Function
VEGFR2 regulates angiogenesis as well as vascular development and permeability
Interaction
VEGF-A, SHC2, NPR1, NCK1
Ligand/Receptor
VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. DiSalvo J, et al. 1995. J. Biol. Chem. 270:7717.
2. McColl BK, et al. 2003. J. Exp. Med. 198:863.
3. Albuquerque RJ, et al. 2009. Nat. Med. 15:1023.
4. Guangqi E, et al. 2012. J. Biol. Chem. 287:3029.
5. Koch S, and Claesson-Welsh 2012. Cold Spring Harb Perspect Med 2:a006502.
6. Munaut C, et al. 2012. Plos One 7:e33475.
7. Wehland M, et al. 2013. Int. J. Mol. Sci. 14:9338.

Gene ID
3791 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 05/09/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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