- TL2.1 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- Toll like receptor 2, TIL4, CD282
- Mouse IgG2a, κ
- Ave. Rating
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- Product Citations
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Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products.Product Details
- Antibody Type
- Host Species
- Human TLR2-transfected CHO cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block, IHC-P, IHC-F, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation. Additional reported applications (for the relevant formats) include: inhibition of PGP activity and blocking of cytokine production1,3,7, immunoprecipitation1, immunohistochemistry of 4% paraformaldehyde-fixed frozen sections2 and immunohistochemistry of HOPE-fixed (HEPES-glutamic acid buffer-mediated organic solvent protection effect) paraffin-embedded sections4 , and Western blotting2. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 309716) (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered).
(PubMed link indicates BioLegend citation)
- Flo T, et al. 2000. J. Immunol. 164:2064.
- Faure E, et al. 2001. J. Immunol. 166:2018.
- Sugawara S, et al. 2001. Infect. Immun. 69:4951.
- Droemann D, et al. 2003. Histochem. Cell Biol. 119:103.
- Chavakis E, et al. 2007. Circ. Res. 100:204.PubMed
- Fiala M, et al. 2007. Proc. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
- Goo SY, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200.PubMed
- Weiss DJ,et al. 2008.J. Leukoc. Biol. 83:48. PubMed
- Harris, KM., et al. 2011. J. Leukoc Biol. 90:727. PubMed.
- Elass-Rocahrd E, et al. 2012. J Biol Chem. 287:34432. PubMed.
- Product Citations
AB_314772 (BioLegend Cat. No. 309702)
- IL-1R superfamily, TLR family, type 1 transmembrane protein, 90 kD
Monocytes, granulocytes, endothelial and epithelial cells
- Activates innate host defense against bacterial pathogens
- Microbial lipoproteins, peptidoglycans, lipoteichoic acid
- Cell Type
- Endothelial cells, Epithelial cells, Granulocytes, Monocytes, Neutrophils, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
1. Lien E, et al. 1999. J. Biol. Chem. 274:33419.
2. Lien E, et al. 2001. J. Biol. Chem. 276:1873.
3. Sabroe I, et al. 2002. J. Immunol. 168:4701
- Gene ID
- 7097 View all products for this Gene ID
- View information about CD282 on UniProt.org
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