- NAT105 (See other available formats)
- Regulatory Status
- Other Names
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.Product Details
- Antibody Type
- Host Species
- YT cell line.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-P, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry of paraffin-embedded tissue, a concentration range of 0.1 - 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Tissue Sections: Paraffin-embedded tissues.
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate H.I.E.R. Retrieval Solution (Cat. No. 928501, 928601).
Incubation: 24 hours at 2°C - 4 °C.
Positive Control: Human tonsil and spleen.
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.
We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).
(PubMed link indicates BioLegend citation)
- Roncador G, et al. 2007. Haematologica 92:1059.
- Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
- Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
- Product Citations
AB_2565782 (BioLegend Cat. No. 367402)
- 55 kD type I transmembrane protein.
Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.
- Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
- SHP-1 and SHP-2.
- PD-L1 (CD274) and PD-L2 (CD273).
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.
- Gene ID
- 5133 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Specificity Alt (DOES NOT SHOW ON TDS):
- View information about CD279 on UniProt.org
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