- 12G5 (See other available formats)
- Regulatory Status
- VII 70204
- Other Names
- CXCR4, Fusin
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.Product Details
- Human, African Green, Baboon, Chimpanzee, Cynomolgus, Rhesus, Sooty Mangabey
- Antibody Type
- Host Species
- CP-MAC-infected Sup-T1 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
ICC, CyTOF® - Verified
IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).
(PubMed link indicates BioLegend citation)
- McKnight A, et al. 1997. J. Virol. 71:1692.
- Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
- Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
- Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
- Ullrich CK, et al. 2000. Blood 96:1438. (Block)
- Murga M, et al. 2005. Blood 105:1992. (IF)
- Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
- Isnardi I, et al. 2010. Blood 115:5026. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Fischer T, et al. 2008. PLoS One 3:e4069.
- Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
- Product Citations
AB_314607 (BioLegend Cat. No. 306501)
AB_314608 (BioLegend Cat. No. 306502)
- Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells
- B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
- SDF-1 receptor, coreceptor for X4 HIV-1
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.
- Gene ID
- 7852 View all products for this Gene ID
- View information about CD184 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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