Purified anti-human CD158b (KIR2DL2/L3, NKAT2) Antibody

Pricing & Availability
DX27 (See other available formats)
Regulatory Status
Other Names
CD158b1 (KIR2DL2, p58.2), CD158b2 (KIR2DL3, p58.3), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Mouse IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes stained with purified DX27, followed by anti-mouse IgG FITC
  • DX27_Purified_071607
    Human peripheral blood lymphocytes stained with purified DX27, followed by anti-mouse IgG FITC
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312602 100 µg $95.00
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CD158b is expressed on natural killer cells and a subset of T cells. It is a member of the immunoglobulin superfamily containing two immunoglobulin C2-type domains. Both variants and alternative isoforms of CD158b have been reported. The interaction of CD158b with specific HLA-C antigens on a target cell (HLA-Cw1, HLA-Cw3, HLA-Cw7 alleles, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK cell and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The DX27 monoclonal antibody reacts with a common epitope of KIR2DL2 (CD158b1, p58.2), KIR2DL3 (CD158b2, p58.3), and KIR2DS2 (CD158j, p50.2). Additional reported applications (for the relevant formats) include: restoring the NK cell cytotoxicity1,5.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
  2. Lucas M, et al. 2003. J. Virol. 77:2251.
  3. Goodier M, et al. 2000. J. Immunol. 165:139.
  4. Yawata M, et al. 2002. Immunogenetics 54:543.
  5. Valiante NM, et al. 1997. Immunity 7:739.
Product Citations
  1. Niu C, et al. 2018. Front Immunol. 9:1759. PubMed
  2. Zal B, et al. 2008. J Immunol. 181:5233. PubMed
  3. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
AB_314933 (BioLegend Cat. No. 312602)

Antigen Details

Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, type I membrane protein

NK cells and a subset of T cells

Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-C antigens on target cell
HLA-C antigens such as HLA-Cw1, HLA-Cw3, HLA-Cw7
Cell Type
NK cells, T cells
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Colonna M, et al. 1995. Science 268:405.
2. Uhrburg M, et al. 1997. Immunity 7:753.
3. Wagtmann N, et al. 1995. Immunity 3:801.
4. Dohring C, et al. 1996. Immunogenetics 44:227.
5. Maenaka K, et al. 1999. Structure 7:391.

Gene ID
3803 View all products for this Gene ID
View information about CD158b on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 10/24/2016

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