- 104D2 (See other available formats)
- Regulatory Status
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Mouse IgG1, κ
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- Product Citations
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CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.Product Details
- Human, Cross-Reactivity: Cynomolgus, Cattle (Bovine, Cow)
- Antibody Type
- Host Species
- MOLM-1 megakaryocytic cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
ICC, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunofluorescence microscopy1.
(PubMed link indicates BioLegend citation)
- Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
- Product Citations
AB_314980 (BioLegend Cat. No. 313201)
AB_314981 (BioLegend Cat. No. 313202)
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- c-Kit ligand
- Multiple phosphorylation sites
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.
- Gene ID
- 3815 View all products for this Gene ID
- View information about CD117 on UniProt.org
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