Purified anti-human CCL28 (MEC) Antibody

Pricing & Availability
Clone
J132C3 (See other available formats)
Regulatory Status
RUO
Other Names
Mucosa-associated epithelial chemokine (MEC), CCK1, SCYA28, small inducible cytokine A28
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
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525602 500 µg $305.00
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Description

Human CCL28 was identified in an EST database using a human chemokine consensus sequence. CCL28 is constitutively expressed by mucosal epithelial cells such as exocrine glands, trachea, and colon. Acinar epithelial cells in human and mouse salivary glands are strongly positive for CCL28 by immunostaining, and human saliva and milk contain high concentrations of CCL28. Furthermore, it is known that human and mouse CCL28 have antimicrobial activity attributed to the C-terminus of CCL28 against Candida albicans, Gram-negative bacteria, and Gram-positive bacteria. In addition, using a CCR10 deficient mouse model, it was shown that CCR10 plays a critical role in localization and accumulation of IgA ASC in lactating mammary glands. Also, it has been described that CCL28 is upregulated in epithelial inflammation, and this upregulation allows the recruitment of Tregs expressing CCR10. In fact, a subpopulation of CCR10-expressing CD25+CD4+FOXP3+ Tregs with potent anti-inflammatory properties was isolated from a chronically inflamed human liver. Information suggests that tumor hypoxia promotes the recruitment of Tregs through induction of expression of CCL28, and this induction is mediated by the hypoxia inducible factor-1α (HIF-1α). Therefore, tumor hypoxia promotes tolerance and angiogenesis via CCL28 and Tregs.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL28
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL28 recombinant protein ranging from 1000 to 15.6 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified J132C3 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated J139G11 antibody (Cat. No. 525703) as the detection antibody.

Note: For testing human CCL28 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human CCL28 ELISA Kit with Pre-coated Plates (Cat. No. 441207 & 441208) are specially developed and recommended.

RRID
AB_2563257 (BioLegend Cat. No. 525602)

Antigen Details

Structure
Chemokine
Distribution

Mucosal epithelial cells, primary human cholangiocytes

Function
CCL28 chemoattracts resting CD4, CD8, and IgA antibody secreting cells. It plays a role in extravasation of IgA ASC in the small intestine, colon, lactating mammary glands, and has antimicrobial activity. CCL28 is upregulated by IL-1 or n-butirate in human colon epithelial cell lines, and IL-1 and n-butirate have a synergistic effect on CCL28 upregulation. Hypoxia induces CCL28 in tumors.
Interaction
CD4 and CD8 T cells, IgA antibody secreting cells, CCR10-expressing CD25+ CD4+ Foxp3+ Tregs, CCR3 expressing eosinophils
Ligand/Receptor
CCR10, CCR3
Cell Type
B cells, Eosinophils, Epithelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Wang W, et al. 2000. J. Biol. Chem. 275:22313.
2. Hieshima K, et al. 2003. J. Immunol. 170:1452.
3. Hieshima K, et al. 2004. J. Immunol. 173:3668.
4. Ogawa H, et al. 2004. Am. J. Physiol. Gastrointest. Liver Physiol. 287:G1062.
5. Eksteen B, et al. 2006. J. Immunol. 177:593.
6. Morteau O, et al. 2008. J. Immunol. 181:6309.
7. Liu B and Wilson E. 2010. Eur. J. Immunol. 40:186.
8. Facciabene A, et al. 2011. Nature 475:226.

Gene ID
56477 View all products for this Gene ID
UniProt
View information about CCL28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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