Purified anti-FITC (Maxpar® Ready) Antibody

Pricing & Availability
FIT-22 (See other available formats)
Regulatory Status
Other Names
Fluorescein isothiocyanate
Mouse IgG1, λ
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Product Citations
Human PBMC stained with FITC-anti-CD3 (UCHT1) then detected with 174Yb anti-FITC (FIT-22). Plotted against 154Sm-anti-CD45 (HI30). Data provided by DVS Sciences.
  • FIT22_Purified_FITC_Cytof_061014.jpg
    Human PBMC stained with FITC-anti-CD3 (UCHT1) then detected with 174Yb anti-FITC (FIT-22). Plotted against 154Sm-anti-CD45 (HI30). Data provided by DVS Sciences.
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408305 100 µg $120.00
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The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
FITC conjugated mouse IgG.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
The antibody was purified by affinity chromatography.
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
Product Citations
  1. Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
AB_2563769 (BioLegend Cat. No. 408305)

Antigen Details

Gene ID
Specificity (DOES NOT SHOW ON TDS):
Specificity Alt (DOES NOT SHOW ON TDS):
View information about FITC on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.


Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 0    Revision Date: 07/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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