Purified anti-CD49C (Integrin α3) Antibody

Pricing & Availability
Clone
P1B5 (See other available formats)
Regulatory Status
RUO
Other Names
GAP-B3, GAPB3, ILNEB, MSK18, VCA-2, VL3A, VLA3a, Integrin alpha-3, FRP-2, galactoprotein B3, VLA-3 subunit alpha, CD49 antigen-like family member C, antigen identified by monoclonal antibody J143, alpha-3 subunit
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
P1B5_Purified_CD49C_Antibody_1_041719.png
IHC staining of purified anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Purified_CD49C_Antibody_1_041719.png
    IHC staining of purified anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Purified_CD49C_Antibody_2_041719.png
    ICC staining of purified anti-CD49C (Integrin α3) antibody (clone P1B5) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. Nuclei were counterstained with DAPI, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • P1B5_Purified_CD49C_Antibody_3_041719.png
    HeLa cells were fixed with 4% PFA, permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002), and stained with purified anti-CD49C (Integrin α3) antibody (clone P1B5, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE(Cat. No. 405307).
Cat # Size Price Quantity Check Availability Save
865201 25 µg $90.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
865202 100 µg $235.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1B5 monoclonal antibody was generated against human HT1080 cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
FC, ICC - Verified
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For flow cytometric staining, the suggested use of this reagent is between 1.0 - 10 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes the a3 subunit.

Application References

(PubMed link indicates BioLegend citation)
  1. Wayner EA and Hoffstrom BG. 2007. Methods. Enzymol. 426:117.
  2. Wayner EA, et al. 1993. J Cell Biol. 121:1141. (IP)
  3. Carter WG, et al. 1990. J Cell Biol. 110:1387-404 (IP)
  4. Wayner EA and Carter WG. 1987. J Cell Biol. 105:1873. (IP)
Product Citations
RRID
AB_2810769 (BioLegend Cat. No. 865201)
AB_2810770 (BioLegend Cat. No. 865202)

Antigen Details

Structure
CD49c is a 150 kD type I transmembrane glycoprotein.
Distribution

Cellular distribution: Plasma membrane.
Tissue distribution: Endothelial cells, epithelial cells, and dermal fibroblast.

Function
CD49c plays a role in cell-cell and cell-matrix adhesion.
Ligand/Receptor
Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
3675 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD49C
Specificity Alt (DOES NOT SHOW ON TDS):
CD49C
UniProt
View information about CD49C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account