Purified anti-Biotin (Maxpar® Ready) Antibody

Pricing & Availability
Clone
1D4-C5 (See other available formats)
Regulatory Status
RUO
Other Names
Vitamin B7, Vitamin H
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1D4-C5_Purified_Biotin_Cytof_060304.jpg
Human PBMCs stained with Biotin-anti Human CD3 (UCHT1) then detected with 143Nd-anti-Biotin (1D4-C5). Plotted against 147Sm-anti-CD20 (2H7). Data provided by DVS Sciences.
  • 1D4-C5_Purified_Biotin_Cytof_060304.jpg
    Human PBMCs stained with Biotin-anti Human CD3 (UCHT1) then detected with 143Nd-anti-Biotin (1D4-C5). Plotted against 147Sm-anti-CD20 (2H7). Data provided by DVS Sciences.
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409007 100 µg $120.00
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Description

Biotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.

Product Details
Technical data sheet

Product Details

Reactivity
All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Biotinylated limulus hemocyanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.

Additional Product Notes

Maxpar® is a registered trademark of Fluidigm Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Uccellini MB, et al. 2008. J. Immunol. 181:5875.
  2. Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.
Product Citations
  1. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
RRID
AB_2563707 (BioLegend Cat. No. 409007)

Antigen Details

Ligand/Receptor
Avidin, Streptavidin
Gene ID
NA
UniProt
View information about Biotin on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 0    Revision Date: 07/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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