Purified anti-Acetyl-CoA Carboxylase 1 Antibody

Pricing & Availability
Clone
W19216A (See other available formats)
Regulatory Status
RUO
Other Names
Acetyl-CoA Carboxylase Alpha, Acetyl-Coenzyme A Carboxylase Alpha, ACC1, ACCA
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19216A_PURE_Acetyl-CoA-Carboxylase_Antibody_110520.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-acetyl-CoA carboxylase 1 antibody (clone W19216A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W19216A_PURE_Acetyl-CoA-Carboxylase_Antibody_110520.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-acetyl-CoA carboxylase 1 antibody (clone W19216A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W19216A_PURE_Acetyl-CoA-Carboxylase_Antibody_2_110520.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) (panel A) or 1.0 µg/mL purified anti-acetyl-CoA carboxylase 1 antibody (clone W19216A) (panel B) followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405422) at 2.0 µg/mL (1:250 dilution). Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • W19216A_PURE_Acetyl-CoA-Carboxylase_Antibody_3_110520.png
    Whole cell extracts (250 μg total protein) prepared from Hep G2 cells were immunoprecipitated overnight with 2.5 μg purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-acetyl-CoA carboxylase 1 antibody (clone W19216A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with W19216A. Lane M: Molecular weight marker.
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942201 25 µg $100.00
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942202 100 µg $250.00
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Description

Acetyl-CoA carboxylase (ACC) is a cytosolic enzyme that catalyzes carboxylation of acetyl-CoA to malonyl-CoA, the initiating and rate-limiting step in fatty acid biosynthesis. Two isoforms of ACC exist that show tissue-specific distribution. ACC1 is predominantly expressed in lipogenic tissues, while ACC2 is found in oxidative tissues. In response to depletion of cellular ATP, AMPK phosphorylates ACC1 at Ser79, resulting in loss of enzymatic activity. ACC1 is a clinical target for various metabolic diseases, and also contributes to metabolic reprogramming in tumor cells.

 

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human acetyl-CoA carboxylase 1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

-Clone W19216A has a weak affinity for mouse acetyl-CoA carboxylase 1; we therefore do not recommend this clone for detecting mouse acetyl-CoA carboxylase

-This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either Triton X-100 or methanol. Both methods were compatible with staining of acetyl-CoA carboxylase 1.

-This clone may cross react with acetyl-CoA carboxylase 2.

RRID
AB_2888906 (BioLegend Cat. No. 942201)
AB_2888906 (BioLegend Cat. No. 942202)

Antigen Details

Structure
Acetyl-CoA carboxylase 1 is a 2,346 amino acid protein with a predicted molecular weight of 266 kD.
Distribution

Lipogenic tissue/Cytosol

Function
Fatty acid biosynthesis
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References

1. Chen L, et al. 2019. Expert Opin. Investig. Drugs. 28:917.
2. Munday MR, et al. 1999. Adv. Enzyme Regul. 39:205.
3. Saddik M, et al. 1993. J Biol Chem. 268:25836.

Gene ID
31 View all products for this Gene ID
UniProt
View information about Acetyl-CoA Carboxylase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/05/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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