PerCP/Cyanine5.5 anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
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Product Citations
publications
4B10_PerCPCyanine55_T-Bet_Antibody_1_012519
Human peripheral blood lymphocytes were surface stained with CD3 FITC, treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), then stained with T-bet (clone 4B10) PerCP/Cyanine5.5 (left) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
  • 4B10_PerCPCyanine55_T-Bet_Antibody_1_012519
    Human peripheral blood lymphocytes were surface stained with CD3 FITC, treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), then stained with T-bet (clone 4B10) PerCP/Cyanine5.5 (left) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
644805 25 µg $170.00
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644806 100 µg $350.00
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Hurton L, et al. 2016. Proc Natl Acad Sci U S A. 113: E7788 - E7797. PubMed
  2. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  3. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  4. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  5. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  6. Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005817. PubMed
  7. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  8. Frasca D, et al. 2019. PLoS One. 14:e0219545. PubMed
  9. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  10. Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed
  11. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  12. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
RRID
AB_1595593 (BioLegend Cat. No. 644805)
AB_1595488 (BioLegend Cat. No. 644806)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
T-bet
Specificity Alt (DOES NOT SHOW ON TDS):
T-bet
UniProt
View information about T-bet on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 5    Revision Date: 01/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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