- YTS156.7.7 (See other available formats)
- Regulatory Status
- Other Names
- Lyt-3, Ly-3
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.Product Details
- Antibody Type
- Host Species
- Mouse thymocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
- Product Citations
AB_961304 (BioLegend Cat. No. 126609)
AB_2260149 (BioLegend Cat. No. 126610)
- Ig superfamily, CD8β chain, 34 kD
Majority of thymocytes, cytotoxic T cell subsets
- Co-receptor of TCR
- MHC class I
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
- Gene ID
- 12526 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Specificity Alt (DOES NOT SHOW ON TDS):
- View information about CD8b on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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