PerCP/Cyanine5.5 anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1_PerCPCyanine55_CD80_Antibody_101818
LPS-stimulated (day 3) BALB/c splenocytes were stained with CD80 (clone 16-10A1) PerCP/Cyanine5.5 (filled histogram), or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
  • 16-10A1_PerCPCyanine55_CD80_Antibody_101818
    LPS-stimulated (day 3) BALB/c splenocytes were stained with CD80 (clone 16-10A1) PerCP/Cyanine5.5 (filled histogram), or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
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104721 25 µg $100.00
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104722 100 µg $275.00
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Cross-Reactivity: Dog (Canine)
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Zhao F et al. 2018. Immunity. 48(1):147-160 . PubMed
  2. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  3. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  4. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  5. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  6. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  7. Argüello RJ, et al. 2018. J Cell Sci. 131:jcs214346. PubMed
  8. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  9. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  10. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  11. Darling R, et al. 2020. Int J Nanomedicine. 5.19375. PubMed
  12. Taylor J, et al. 2012. J Exp Med. 209:2065. PubMed
  13. Luger R, et al. 2013. PLoS One. 8:e54879. PubMed
  14. Wallner S, et al. 2013. PLoS One. 8:65178. PubMed
  15. Alves C, et al. 2015. PLoS One. 10: 0142972. PubMed
  16. Collinson-Pautz M, et al. 2016. PLoS One. 11:e0164547. PubMed
  17. OlguÍn-Alor R, et al. 2016. PLoS One. 11:e0167813. PubMed
  18. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  19. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  20. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  21. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  22. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
RRID
AB_893406 (BioLegend Cat. No. 104721)
AB_2291392 (BioLegend Cat. No. 104722)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10/18/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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