PerCP/Cyanine5.5 anti-mouse CD71 Antibody

Pricing & Availability
Clone
RI7217 (See other available formats)
Regulatory Status
RUO
Other Names
Transferrin receptor, T9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RI7217_PerCPCy5.5_CD71_Antibody_FC_1_022015
C57BL/6 mouse bone marrow cells were stained with Ter-119 APC and CD71 (clone RI7217) PerCP/Cyanine5.5 (top) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (bottom). Data shown was gated on total bone marrow cells.
  • RI7217_PerCPCy5.5_CD71_Antibody_FC_1_022015
    C57BL/6 mouse bone marrow cells were stained with Ter-119 APC and CD71 (clone RI7217) PerCP/Cyanine5.5 (top) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (bottom). Data shown was gated on total bone marrow cells.
  • RI7217_PerCPCy5.5_CD71_Antibody_FC_2_022015
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
113815 25 µg $125.00
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113816 100 µg $275.00
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Description

CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of cellular proliferation. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 113821 & 113822).

This clone may also be known as R17217 or R17 217.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge I, et al. 1982. J. Cell. Physiol. 112:403.
  2. Grisendi S, et al. 2005. Nature 437:147.
  3. van Rooy I, et al. 2010. J. Control Release 150:30. PubMed
  4. Willhelm BT, et al. 2011. Blood 117:27. PubMed
  5. Okasi Y, et al. 2012. Exp Hematol. 40:143PubMed
  6. Stojanov K, et al. 2012. Mol Pharm. 9:1620. PubMed
Product Citations
  1. Long M, et al. 2017. J Immunol. 198(2):862-872. PubMed
  2. Lissner MM, et al. 2020. Elife. 9:00. PubMed
RRID
AB_2565481 (BioLegend Cat. No. 113815)
AB_2565482 (BioLegend Cat. No. 113816)

Antigen Details

Structure
Transferrin receptor and is 95 kD.
Distribution

Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.

Function
Mediates iron uptake.
Ligand/Receptor
Transferrin.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95.

Gene ID
22042 View all products for this Gene ID
UniProt
View information about CD71 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 02/20/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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