PerCP/Cyanine5.5 anti-human CD305 (LAIR1) Antibody

Pricing & Availability
Clone
NKTA255 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LAIR-1 (Leukocyte-associated Ig-like receptor 1)
Isotype
Mouse IgG1
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Product Citations
publications
NKTA255_PerCPCyanine55_CD305_Antibody_1_060719
Human peripheral blood lymphocytes were stained with CD305 (LAIR1) (clone NKTA255) PerCP/Cyanine5.5 (filled histogram), or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • NKTA255_PerCPCyanine55_CD305_Antibody_1_060719
    Human peripheral blood lymphocytes were stained with CD305 (LAIR1) (clone NKTA255) PerCP/Cyanine5.5 (filled histogram), or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
See PerCP/Cyanine5.5 spectral data
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342804 100 tests $325.00
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Description

CD305, also known as LAIR-1 (leukocyte-associated Ig-like receptor-1), is a 40 kD type I transmembrane glycoprotein. LAIR-1 and LAIR-2 are the only members of the LAIR family in the Ig superfamily. LAIR-1 is an inhibitory molecule characterized by ITIMs in the cytoplasmic domain and collagen ligands. CD305 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells. LAIR-1 functions in the inhibition of cell cytotoxicity, cell activation, proliferation and differentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Poggi A, et al. 1995. Eur. J. Immunol. 25:369
RRID
AB_2133478 (BioLegend Cat. No. 342804)

Antigen Details

Structure
40 kD type I transmembrane protein, cytoplasmic domain contains ITIM motifs, Ig superfamily
Distribution

NK cells, T cell, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells

Function
Inhibition of cell cytotoxicity, cell activation, proliferation and differentiation
Ligand/Receptor
collagens
Cell Type
B cells, Basophils, Dendritic cells, Eosinophils, Macrophages, Mast cells, Monocytes, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Meyaard L, et al. 2008. J. Leukoc. Biol. 83:799
2. Lebbink RJ, et al. 2006. J. Exp. Med. 203:1419

Gene ID
3903 View all products for this Gene ID
UniProt
View information about CD305 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06/07/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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