PerCP/Cyanine5.5 anti-human GARP (LRRC32) Antibody

Pricing & Availability
Clone
7B11 (See other available formats)
Regulatory Status
RUO
Other Names
Garpin, Glycoprotein A repetitions predominant
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
7B11_PerCPCy55_GARP_Antibody_042518
Human peripheral blood mononuclear cells were stimulated with CD3, CD28 and recombinant human IL-2 for 24 hours and then stained with CD4 APC, LAP (TGF-ß) FITC and GARP (clone 7B11) PerCP/Cyanine5.5 (left) or mouse IgG2b, ? PerCP/Cyanine5.5 isotype control (right). Plots shown are gated on CD4-positive cells.
  • 7B11_PerCPCy55_GARP_Antibody_042518
    Human peripheral blood mononuclear cells were stimulated with CD3, CD28 and recombinant human IL-2 for 24 hours and then stained with CD4 APC, LAP (TGF-ß) FITC and GARP (clone 7B11) PerCP/Cyanine5.5 (left) or mouse IgG2b, ? PerCP/Cyanine5.5 isotype control (right). Plots shown are gated on CD4-positive cells.
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
352513 25 tests $135.00
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352514 100 tests $325.00
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Description

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LRRC32-DNA vaccination
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
RRID
AB_2734371 (BioLegend Cat. No. 352513)
AB_2734372 (BioLegend Cat. No. 352514)

Antigen Details

Structure
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution

Activated Tregs, megakaryocytes, platelets

Ligand/Receptor
LAP (TGF-β1)
Cell Type
Megakaryocytes, Platelets, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213.
2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315.
3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439.
4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.

Gene ID
2615 View all products for this Gene ID
UniProt
View information about GARP on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 04/26/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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