PerCP anti-rat CD62L Antibody

Pricing & Availability
Clone
OX-85 (See other available formats)
Regulatory Status
RUO
Other Names
LECAM-1, Ly-22, LAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-85_PerCP_011909.jpg
LOU rat splenocytes stained with OX-85 PerCP and anti-rat CD3 (1F4) Alexa Fluor® 647
  • OX-85_PerCP_011909.jpg
    LOU rat splenocytes stained with OX-85 PerCP and anti-rat CD3 (1F4) Alexa Fluor® 647
See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
202914 100 µg $250.00
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Description

CD62L is a 74-95 kD glycoprotein,also known as L-selectin, LECAM-1, Ly-22 and MEL-14.It is a member of the selectin family and is expressed on the majority of B and naïve T cells,a subset of memory T cells,granulocytes ,subsets of thymocytes and NK cells.CD62L is important in lymphocyte homing to high endothelial venules in peripheral lymph nodes and leukocyte “rolling” on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites.CD62L is rapidly shed from lymphocytes and neutrophils upon activation and the expression levels of CD62L (in conjunction with other markers) hae been used to distinguish naïve, effector and memory T cells.CD62L has been reported to interact with )-glycans presented on CD34, GlyCAM-1, and MadCAM-1.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat purified CD62L-CD4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vosine C. 2002 J. Immunol. 169:2284.
Product Citations
  1. Palmieri D, et al. 2011. J Surg Res. 171:237. PubMed
RRID
AB_2185083 (BioLegend Cat. No. 202914)

Antigen Details

Structure
74-95 Kd glycoprotein of selectin family.
Distribution

Expressed on majority of B cell and naïve T cell and other leukocytes

Function
Homing and emigration of leukocytes
Cell Type
B cells, T cells, Leukocytes
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Regulation
Decreased expression upon cell activation
Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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