PerCP anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_PerCP_011508
C57BL/6 mouse splenocytes stained with 30-F11 PerCP
  • 30-F11_PerCP_011508
    C57BL/6 mouse splenocytes stained with 30-F11 PerCP
See PerCP spectral data
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103129 25 µg $90.00
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103130 100 µg $245.00
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
  2. Enam S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.013. PubMed
  3. Peñaloza HF, et al. 2018. Biochem Biophys Rep. 13:12. PubMed
  4. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  5. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  6. Oldrini B, et al. 2018. Nat Commun. 9:1466. PubMed
  7. Rangaraju S, et al. 2018. Mol Neurodegener. 13:24. PubMed
  8. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  9. Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed
  10. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
  11. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  12. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  13. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
  14. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  15. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  16. Chen R, et al. 2019. Mol Med Rep. 1.172916667. PubMed
  17. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
  18. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  19. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  20. Wang F, et al. 2019. Cell Death Dis. 1.036111111. PubMed
  21. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  22. Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed
  23. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  24. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  25. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  26. She L, et al. 2019. J Immunol. 203:2520. PubMed
  27. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  28. Wong L, et al. 2013. J Biol Chem. 288:35170. PubMed
  29. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  30. West D, et al. 2014. J Immunol. 192:1762. PubMed
  31. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  32. Yamasaki R, et al. 2014. J Exp Med. 211:1533. PubMed
  33. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  34. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  35. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  36. Yin X, et al. 2015. J Immunol. 194:4029. PubMed
  37. Woods S, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:912. PubMed
  38. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
  39. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  40. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
  41. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
  42. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  43. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  44. Lopez-Guadamillas E, et al. 2016. Sci Rep. 6:34542. PubMed
  45. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
  46. Tajfirouz D, et al. 2017. Virology. 10.1016/j.virol.2017.02.022. PubMed
  47. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  48. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  49. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
  50. Yamamuro T, et al. 2020. Nat Commun. 3.340277778. PubMed
  51. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  52. Demandt JAF, et al. 2021. Frontiers in Cell and Developmental Biology. 9:664258. PubMed
  53. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  54. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  55. Cao Z, et al. 2021. Molecular Cell. 81(17):3604-3622.e10. PubMed
  56. Sun J, et al. 2020. Acta Biomater. 289:106. PubMed
  57. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  58. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  59. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  60. Doni A, et al. 2021. Nat Commun. 12:3739. PubMed
RRID
AB_893343 (BioLegend Cat. No. 103129)
AB_893339 (BioLegend Cat. No. 103130)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 05/14/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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