PE/Cyanine7 anti-human CD51 Antibody

Pricing & Availability
Clone
NKI-M9 (See other available formats)
Regulatory Status
RUO
Workshop
IV 103
Other Names
Vitronectin receptor α chain, Integrin αV, αV integrin, ITGAV
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
NKI-M9_PECyanine7_CD51_Antibody_070720
Human melanoma cell line M21 was stained with CD51 (clone NKI-M9) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • NKI-M9_PECyanine7_CD51_Antibody_070720
    Human melanoma cell line M21 was stained with CD51 (clone NKI-M9) PE/Cyanine7 (filled histogram) or mouse IgG2a, κ PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
327915 25 tests $125.00
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327916 100 tests $260.00
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Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin αV. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Melonama cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Defilippi P, et al. 1991. J. Cell Biol. 114:855. PubMed
  3. Burdick MM, et al. 2003. Am. J. Physiol.Cell Ph. 284:C977. PubMed
  4. Grzeszkiewicz TM, et al. 2001. J. Biol. Chem. 276:21943.
  5. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145. PubMed
  6. Silverman AP, et al. 2008. J Mol Biol. 385:1064. PubMed
  7. Khurana S, et al. 2013. Blood. 121:2587. PubMed
RRID
AB_2876634 (BioLegend Cat. No. 327915)
AB_2876634 (BioLegend Cat. No. 327916)

Antigen Details

Structure
Integrin, forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8, 125 kD and 24 kD in reduced condition, 150 kD (unreduced)
Distribution

Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Function
Adhesion, angiogenesis
Ligand/Receptor
Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen, fibronectin, laminin.
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication

Gene ID
3685 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/07/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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