PE Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MPC-11 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
400311 25 µg $35.00
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400313 25 tests $35.00
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400314 100 tests $90.00
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400312 100 µg $95.00
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Description

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this mouse IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations
  1. Venet F, et al. 2017. J Immunol. 10.4049/jimmunol.1700127. PubMed
  2. Coleman LG, et al. 2018. PLoS One. 13:e0195335. PubMed
  3. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  4. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  5. Oh JH, et al. 2019. Cancer Immunol Res. 0.697222222. PubMed
  6. Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed
  7. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  8. Ibuki M, et al. 2019. Regen Ther. 0.551388889. PubMed
  9. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  10. Yang GY, et al. 2020. Vet Res. 51:26. PubMed
  11. Berger V, et al. 2013. Protein Eng Des Sel. 26:581. PubMed
  12. Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
  13. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  14. Guan H, et al. 2016. Sci Rep. 6:35651. PubMed
  15. Santiago L, et al. 2020. Cell Rep. 107847:32. PubMed
  16. Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed
  17. Tang S, et al. 2021. Front Cell Dev Biol. 9:670913. PubMed

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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