PE/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PECy7_083105
C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
  • 17A2_PECy7_083105
    C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
See PE/Cyanine7 spectral data
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100219 25 µg $95.00
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100220 100 µg $225.00
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Product Citations
  1. Patnode M, et al. 2013. J Biol Chem. 288:26533. PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  4. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  5. M H, et al. 2016. Sci Rep. 6: 20588. PubMed
  6. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  7. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  8. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  9. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  10. Eastman AE, et al. 2020. Cell Rep. 31:107804. PubMed
  11. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  12. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  13. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  14. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  15. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  16. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  17. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  18. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  19. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  20. Vacca M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01462. PubMed
  21. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  22. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  23. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  24. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  25. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  26. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  27. Chen Z, et al. 2019. Mol Med Rep. 19:3210. PubMed
  28. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  29. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  30. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  31. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  32. Page N, et al. 2018. Immunity. 48:937. PubMed
  33. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  34. Schneider C, et al. 2018. Cell. 174:271. PubMed
  35. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  36. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  37. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  38. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  39. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  40. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  41. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  42. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  43. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  44. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
  45. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  46. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  47. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
RRID
AB_1732068 (BioLegend Cat. No. 100219)
AB_1732057 (BioLegend Cat. No. 100220)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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