PE/Cyanine7 anti-mouse CD159a (NKG2AB6) Antibody

Pricing & Availability
Clone
16A11 (See other available formats)
Regulatory Status
RUO
Other Names
KLRC1, NKG2B, NKG2AB6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
16A11_PECy7_CD159a_Antibody_031518
C57BL/6 mouse splenocytes were stained with TruStain fcX™ (clone 93), then True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-mouse NK-1.1 (clone PK136) APC, and anti-mouse CD159a (clone 16A11) PE/Cyanine7 (left) or Mouse IgG2b, ? Isotype control PE/Cyanine7 (right).
  • 16A11_PECy7_CD159a_Antibody_031518
    C57BL/6 mouse splenocytes were stained with TruStain fcX™ (clone 93), then True-Stain Monocyte Blocker™ (Cat. No. 426103), anti-mouse NK-1.1 (clone PK136) APC, and anti-mouse CD159a (clone 16A11) PE/Cyanine7 (left) or Mouse IgG2b, ? Isotype control PE/Cyanine7 (right).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
142809 25 µg $115.00
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142810 100 µg $285.00
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Description

CD159a, also known as NKG2A or KLRC1 (killer cell lectin-like receptor subfamily C, member 1), is a 43 kD type II transmembrane protein with extracellular C-type lectin domains.  It belongs to the killer cell lectin-like receptor family also known as the NKG2 family.  It is expressed on NK and NKT cells and activated CD8+ T cells.  NKG2A binds to non-classical MHC class I molecule Qa-1 and causes inhibition of NK cell-mediated target-cell lysis. 

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/6 mouse CD94/NKG2A transfected CHO cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use licenses cannot be guaranteed from the University of California.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
  2. Vance RE, et al. 2002. P. Natl. Acad. Sci. USA 99:868. (FC)
RRID
AB_2728160 (BioLegend Cat. No. 142809)
AB_2728161 (BioLegend Cat. No. 142810)

Antigen Details

Structure
Type II transmembrane protein with extracelluar C-type lectin domains, complexed with CD94; 43 kD
Distribution

NK cells and NK T cells from C57BL/6 mouse

Function
Inhibitory activity via ITIMs
Ligand/Receptor
Non-classical MHC-I molecule Qa-1
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, NK cells, NKT cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, MHC Antigens
Antigen References

1. Vance RE, et al. 1998. J. Exp. Med. 188:1841.
2. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
3. Sivakumar PV, et al. 1999. J. Immunol. 162:6976.
4. Silver ET, et al. 1999. Immunogenetics 49:727.
5. Vance RE, et al. 1999. J. Exp. Med. 190:1801.

Gene ID
16641 View all products for this Gene ID
UniProt
View information about CD159a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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