PE anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
h57-597
C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC
  • h57-597
    C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC
See PE spectral data
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109207 50 µg $60.00
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109208 200 µg $180.00
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  2. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  3. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  4. Thilakasiri P, et al. 2019. EMBO Mol Med. 11:4. PubMed
  5. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  6. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  7. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  8. Young A, et al. 2017. Cancer Res. 77:4684. PubMed
  9. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  10. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  11. Tanno H, et al. 2020. Sci Adv. 6:eaay9093. PubMed
  12. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  13. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  14. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  15. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
  16. Tung J, et al. 2013. Nutrition. 20:9007. PubMed
  17. Mahlios J, Zhuang Y 2011. Mol Immunol. 49:227. PubMed
  18. Wolf B, et al. 2015. J Immunol. 195: 2540-2551. PubMed
  19. Beil-Wagner J, et al. 2016. Sci Rep. 6:21377. PubMed
  20. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  21. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  22. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  23. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  24. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  25. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  26. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  27. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  28. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  29. Ding R, et al. 2021. Theranostics. 4957:11. PubMed
  30. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  31. Molgora M, et al. 2020. Cell. 182:886. PubMed
  32. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
RRID
AB_313430 (BioLegend Cat. No. 109207)
AB_313431 (BioLegend Cat. No. 109208)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/06/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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