- S16009B (See other available formats)
- Regulatory Status
- Other Names
- Prf1, Pfp, Pfn, Pfn-1
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.Product Details
- Antibody Type
- Host Species
- Mouse perforin transfected cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains.
- Product Citations
AB_2721640 (BioLegend Cat. No. 154405)
AB_2721641 (BioLegend Cat. No. 154406)
- 70 kD Protein that oligomerizes for pore formation.
CTL, NK (Cytoplasmic Granules)
- Target cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Molecular Family
- Antigen References
- Podack ER, et al, 1988, Immunol Rev, 103:203
- Konjar S, et al, 2010. Immunology, 131:257
- Trapani JA, et al, 2002, Nat Rev Immunol, 2:735
- Osinska I, et al. 2014, Cent Eur J Immnol, 39:109
- Gene ID
- 18646 View all products for this Gene ID
- View information about Perforin on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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