PE anti-mouse Ly-49AB6 Antibody

Pricing & Availability
Clone
A1/Ly49A (See other available formats)
Regulatory Status
RUO
Other Names
Ly49A
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A1slashLy49A_PE_060210
C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1 APC
  • A1slashLy49A_PE_060210
    C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1 APC
See PE spectral data
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138703 25 µg $70.00
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Description

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The A1/Ly49A antibody preferentially reacts with CRD of Ly-49AB6 alloantigen and has also shown cross-reaction with Ly-49ANOD, Ly-49PNOD and Ly-49P129/J.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse C57BL/6N T lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagasawa R, et al. 1987. J. Immunol. 138:815. (IP)
  2. Guy-Grand D, et al. 1996. Eur. J. Immunol. 26:2248. (FC)
  3. Zimmer J, et al. 2001. J. Exp. Med. 194:1531. (FC)
Product Citations
  1. Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed
RRID
AB_2134787 (BioLegend Cat. No. 138703)

Antigen Details

Structure
C-type lectin, Ly-49 family, ITIM, 44 kD
Distribution

NK cells, NKT cells

Function
Inhibitory NK cell receptor
Ligand/Receptor
H-2Dd, H-2Dk, H-2Dp
Cell Type
NK cells, NKT cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Silver ET, et al. 2000. J. Immunol. 165:1771.

Gene ID
16627 View all products for this Gene ID
UniProt
View information about Ly-49AB6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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