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PE anti-mouse/human Bcl-6 Antibody

Pricing & Availability
Clone
IG191E/A8 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell lymphoma 6, BCL-5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
IG191EslashA8_PE_1_060812
Lymph node cells from a C57BL/6 mouse, at day 12, post-immunization, were surface-stained with CD19 PE/Cy7 and IgD PerCP/Cy5.5. They were then fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and stained with Bcl-6 (clone IG191E/A8) PE. Data shown was gated on CD19+ positive cells.
  • IG191EslashA8_PE_1_060812
    Lymph node cells from a C57BL/6 mouse, at day 12, post-immunization, were surface-stained with CD19 PE/Cy7 and IgD PerCP/Cy5.5. They were then fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and stained with Bcl-6 (clone IG191E/A8) PE. Data shown was gated on CD19+ positive cells.
  • IG191EslashA8_PE_2_060812
    Overlay between germinal center (IgD-/Fas+, filled histogram) and naïve (IgD+/Fas-, open histogram) B cell populations.
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648303 25 tests $153
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648304 100 tests $396
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Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family.  It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Plasmid vector containing cDNA of the Bcl-6 gene
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone is also known as GI191E/A8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin fixed, paraffin embedded tissue sections1, Western blotting1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Garcfa JF, et al. 2006. J. Histochem Cytochem. 54:31.
Product Citations
  1. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  2. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  3. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  4. Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed
  5. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
RRID
AB_2561374 (BioLegend Cat. No. 648303)
AB_2561375 (BioLegend Cat. No. 648304)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Ye BH, et al. 1993. Science 262:747.
2. Baron BW, et al. 1993. P. Natl. Acad. Sci. USA 90:5262.
3. Onizuka T, et al. 1995. Blood 86:28.
4. Johnston RJ, et al. 2009. Science 325:1006.
5. Kitano M, et al. 2011. Immunity 34:961.
6. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
7. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID 12053 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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