PE anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20
SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
  • A20
    SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
See PE spectral data
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110707 50 µg $65.00
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110708 200 µg $180.00
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  2. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  3. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  4. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  5. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  6. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  7. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  8. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  9. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  10. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  11. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  12. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  13. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  14. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  15. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  16. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  17. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  18. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  19. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  20. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  21. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  22. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  23. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  24. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  25. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  26. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  27. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  28. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  29. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  30. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  31. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  32. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  33. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  34. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  35. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  36. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  37. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  38. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  39. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  40. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  41. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  42. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  43. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  44. Danzer H, et al. 2020. Elife. 9:00. PubMed
  45. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  46. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  47. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  48. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  49. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  50. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  51. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  52. Sano T, et al. 2021. Cell Reports. 36(8):109608. PubMed
  53. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  54. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  55. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  56. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  57. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
RRID
AB_313496 (BioLegend Cat. No. 110707)
AB_313497 (BioLegend Cat. No. 110708)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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