PE anti-mouse CD126 (IL-6Rα) Antibody

Pricing & Availability
Clone
W18166A (See other available formats)
Regulatory Status
RUO
Other Names
Interluekin 6 receptor alpha, CD126
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18166A_PE_CD126_IL-6Ralpha_Antibody_062921.png
C57BL/6 splenocytes were stained with anti-mouse CD3 FITC and anti-mouse CD126 (clone W18166A) PE (left) or rat IgG2a, κ PE isotype control (right).
  • W18166A_PE_CD126_IL-6Ralpha_Antibody_062921.png
    C57BL/6 splenocytes were stained with anti-mouse CD3 FITC and anti-mouse CD126 (clone W18166A) PE (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
Cat # Size Price Quantity Avail. Save
160405 25 µg $95
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Description

CD126 (IL-6Rα) is an 80kD member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IL-6R recombinant protein, 1-357 a.a.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W18166A binds to IL-6 receptor alpha, inhibits IL-6/IL-6 receptor interaction and mediated phosphorylation of signal transducer and activator of transcription 3 (STAT3). This clone does not cross-react with rat.

Antigen Details

Structure
Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution

T cells and activated B cells, monocytes, hepatocytes, plasma cells

Function
Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor
IL-6
Cell Type
B cells, Epithelial cells, Fibroblasts, Granulocytes, Monocytes, Plasma cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

Taga T, et al. 1997. Annu Rev Immunol. 15:797-819.

Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.

Boulanger MJ, et al. 2003. Science. 300:2101-4

Gene ID
16194 View all products for this Gene ID
UniProt
View information about CD126 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/30/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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