PE anti-human FcεRIα Recombinant Antibody

Pricing & Availability
Clone
QA20A04 (See other available formats)
Regulatory Status
RUO
Other Names
FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A04_PE_FcepsilonRIalpha_Recombinant_Antibody_updated_041521.png
Human peripheral blood lymphocytes were stained with CD203c APC and anti-human FcεRIα recombinant (clone QA20A04) PE (left) or Mouse IgG1, κ PE isotype control (right).
  • QA20A04_PE_FcepsilonRIalpha_Recombinant_Antibody_updated_041521.png
    Human peripheral blood lymphocytes were stained with CD203c APC and anti-human FcεRIα recombinant (clone QA20A04) PE (left) or Mouse IgG1, κ PE isotype control (right).
See PE spectral data
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365303 25 tests $140.00
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Description

High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneous Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2892444 (BioLegend Cat. No. 365303)

Antigen Details

Structure
Ig superfamily, 50 kD
Distribution

Mast cells, basophils, cutaneous Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients

Function
Bind IgE, trigger IgE-mediated allergic response
Ligand/Receptor
IgE
Antigen References
  1. Khodoun M, et al. 2020. J Allergy Clin Immunol. 2020.10.045.
  2. Garman S, et al. 2000. Nature. 406:259–266.
Gene ID
2205 View all products for this Gene ID
UniProt
View information about FcepsilonRIalpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/15/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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