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PE anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
A17188B (See other available formats)
Regulatory Status
RUO
Other Names
PD1, PDCD1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17188B_PE_CD279_Antibody_012820
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD3 APC and anti-human CD279 (PD-1) (clone A17188B) PE (left) or mouse IgG2b, κ PE isotype control (right).
  • A17188B_PE_CD279_Antibody_012820
    PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD3 APC and anti-human CD279 (PD-1) (clone A17188B) PE (left) or mouse IgG2b, κ PE isotype control (right).
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621607 25 tests $146
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621608 100 tests $304
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Description

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD279 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.

Product Citations
  1. Halim L, et al. 2022. Front Immunol. 13:836549. PubMed
  2. Lucas C, et al. 2021. Nature. Online ahead of print. PubMed
RRID
AB_2832827 (BioLegend Cat. No. 621607)
AB_2832828 (BioLegend Cat. No. 621608)

Antigen Details

Structure
55 kD type I transmembrane protein
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling and co-stimulation (co-inhibition)
Interaction
SHP-1 and SHP-2
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887
  2. Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/28/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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