PE anti-human CD183 (CXCR3) Antibody

Pricing & Availability
Clone
G025H7 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
G025H7_PE_1_121911
Human peripheral blood lymphocytes were stained with CD3 APC and CXCR3 (clone G025H7) PE (top plot) or mouse IgG1 PE isotype control (bottom plot).
  • G025H7_PE_1_121911
    Human peripheral blood lymphocytes were stained with CD3 APC and CXCR3 (clone G025H7) PE (top plot) or mouse IgG1 PE isotype control (bottom plot).
  • G025H7_PE_2_121911
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
353705 25 tests $125.00
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353706 100 tests $325.00
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Description

Human CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR3 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  2. Wang W, et al. 2019. Cell Rep. 28:486. PubMed
  3. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  4. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  5. Eckert EC, et al. 2020. Mol Ther Oncolytics. 0.710416667. PubMed
  6. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
  7. Pein M, et al. 2020. Nat Commun. 11:1494. PubMed
  8. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  9. Alexander T, et al. 2013. Ann Rheum Dis. 72:1549. PubMed
  10. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  11. Biasci D, et al. 2020. Proc Natl Acad Sci U S A. 117:28960. PubMed
  12. Bhattacharya P, et al. 2020. Elife. 9:00. PubMed
RRID
AB_10959652 (BioLegend Cat. No. 353705)
AB_10962912 (BioLegend Cat. No. 353706)

Antigen Details

Structure
CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor
Distribution

T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells

Function
Essential in T cell recruitment to sites of inflammation
Ligand/Receptor
CXCL9, CXCL10, and CXCL11
Cell Type
Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Loetscher M, et al. 1996. J. Exp. Med. 184:963.
2. Cole KE, et al. 1998. J. Exp. Med. 187:2009.
3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909.
4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887.
5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089.
6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67.

Gene ID
2833 View all products for this Gene ID
UniProt
View information about CD183 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD183 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD183 (CXCR3) G025H7 FC
APC/Cyanine7 anti-human CD183 (CXCR3) G025H7 FC
FITC anti-human CD183 (CXCR3) G025H7 FC
PE anti-human CD183 (CXCR3) G025H7 FC
APC anti-human CD183 (CXCR3) G025H7 FC
Alexa Fluor® 488 anti-human CD183 (CXCR3) G025H7 FC
Alexa Fluor® 647 anti-human CD183 (CXCR3) G025H7 FC
PerCP/Cyanine5.5 anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 421™ anti-human CD183 (CXCR3) G025H7 FC
PE/Cyanine7 anti-human CD183 (CXCR3) G025H7 FC
Pacific Blue™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 510™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 605™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 650™ anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 711™ anti-human CD183 (CXCR3) G025H7 FC
Purified anti-human CD183 (CXCR3) (Maxpar® Ready) G025H7 FC, CyTOF®
PE/Dazzle™ 594 anti-human CD183 (CXCR3) G025H7 FC
PerCP anti-human CD183 (CXCR3) G025H7 FC
Brilliant Violet 785™ anti-human CD183 (CXCR3) G025H7 FC
Alexa Fluor® 700 anti-human CD183 (CXCR3) G025H7 FC
Biotin anti-human CD183 (CXCR3) G025H7 FC
TotalSeq™-A0140 anti-human CD183 (CXCR3) G025H7 PG
TotalSeq™-C0140 anti-human CD183 (CXCR3) G025H7 PG
Ultra-LEAF™ Purified anti-human CD183 (CXCR3) G025H7 Block, FC
TotalSeq™-B0140 anti-human CD183 (CXCR3) G025H7 PG
APC/Fire™ 750 anti-human CD183 (CXCR3) G025H7 FC
TotalSeq™-D0140 anti-human CD183 (CXCR3) G025H7 PG
PE/Fire™ 810 anti-human CD183 (CXCR3) Antibody G025H7 FC
APC/Fire™ 810 anti-human CD183 (CXCR3) Antibody G025H7 FC
PE/Cyanine5 anti-human CD183 (CXCR3) Antibody G025H7 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD183 (CXCR3)

  • APC/Cyanine7 anti-human CD183 (CXCR3)

  • FITC anti-human CD183 (CXCR3)

  • PE anti-human CD183 (CXCR3)

  • APC anti-human CD183 (CXCR3)

  • Alexa Fluor® 488 anti-human CD183 (CXCR3)

  • Alexa Fluor® 647 anti-human CD183 (CXCR3)

  • PerCP/Cyanine5.5 anti-human CD183 (CXCR3)

  • Brilliant Violet 421™ anti-human CD183 (CXCR3)

  • PE/Cyanine7 anti-human CD183 (CXCR3)

  • Pacific Blue™ anti-human CD183 (CXCR3)

  • Brilliant Violet 510™ anti-human CD183 (CXCR3)

  • Brilliant Violet 605™ anti-human CD183 (CXCR3)

  • Brilliant Violet 650™ anti-human CD183 (CXCR3)

  • Brilliant Violet 711™ anti-human CD183 (CXCR3)

  • Purified anti-human CD183 (CXCR3) (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD183 (CXCR3)

  • PerCP anti-human CD183 (CXCR3)

  • Brilliant Violet 785™ anti-human CD183 (CXCR3)

  • Alexa Fluor® 700 anti-human CD183 (CXCR3)

  • Biotin anti-human CD183 (CXCR3)

  • TotalSeq™-A0140 anti-human CD183 (CXCR3)

  • TotalSeq™-C0140 anti-human CD183 (CXCR3)

  • Ultra-LEAF™ Purified anti-human CD183 (CXCR3)

  • TotalSeq™-B0140 anti-human CD183 (CXCR3)

  • APC/Fire™ 750 anti-human CD183 (CXCR3)

  • TotalSeq™-D0140 anti-human CD183 (CXCR3)

  • PE/Fire™ 810 anti-human CD183 (CXCR3) Antibody

  • APC/Fire™ 810 anti-human CD183 (CXCR3) Antibody

  • PE/Cyanine5 anti-human CD183 (CXCR3) Antibody

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