PE anti-human CD172b (SIRPβ) Antibody

Pricing & Availability
Clone
B4B6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
SIRPb, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B4B6_PE_111407
Human peripheral blood monocytes stained with B4B6 PE
  • B4B6_PE_111407
    Human peripheral blood monocytes stained with B4B6 PE
See PE spectral data
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323906 100 tests $220.00
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Description

The B4B6 antibody recognizes human CD172b also known as signal-regulatory protein beta, and SIRP-beta. CD172b is a member of the signal regulatory protein family and immunoglobulin superfamily. CD172b contains a single pass transmembrane region with immunoglobulin-like domains and unlike CD172a, this receptor lacks a cytoplasmic SHP-2 binding domain. CD172b is a disulfide-linked homodimer with a predicted molecular weight of approximately 43 kD. Two isoforms of CD172b are produced by alternative splicing. CD172b is expressed on myeloid cells including monocytes and dendritic cells where it functions in the negative regulation of tyrosine-kinase coupled signaling processes. CD172b has been shown to regulate neutrophil transepithelial migration. CD172b interacts with DAP12 and Syk kinase (through TYROBP). The B4B6 antibody has been shown to be useful for flow cytometry.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with SIRPbeta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Seiffert M, et al. 2001. Blood 97:2741.
Product Citations
  1. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  2. Bsat M, et al. 2015. J Leukoc Biol. 98: 671 - 681. PubMed
RRID
AB_2254637 (BioLegend Cat. No. 323906)

Antigen Details

Structure
Member of the signal regulatory protein family and immunoglobulin superfamily. Single pass transmembrane receptor with immunoglobulin-like domains; lacks cytoplasmic SHP-2 binding domain. Disulfide-linked homodimer, predicted MW ~43 kD.
Distribution

Myeloid cells, monocytes, dendritic cells

Function
Negative regulation of tyrosine-kinase coupled signaling processes. Regulates neutrophil transepithelial migration
Interaction
DAP12 and Syk (through TYROBP)
Cell Type
Dendritic cells, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kharitonenkov A, et al. 1997. Nature 386:181.
2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147.

Gene ID
10326 View all products for this Gene ID
UniProt
View information about CD172b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10/25/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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