PE anti-human CD140b (PDGFRβ) Antibody

Pricing & Availability
Clone
18A2 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet-derived growth factor receptor, beta polypeptide, PDGFR1, PDGFRβ, PDGFRb, PDGF receptor beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
18A2_PE_022607
Human PDGFRB transfected cells stained with purified 18A2 PE
  • 18A2_PE_022607
    Human PDGFRB transfected cells stained with purified 18A2 PE
See PE spectral data
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323605 25 tests $100
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323606 100 tests $220
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Description

CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 124 kD. CD140b is widely expressed on a variety of mesenchymal-derived cells and is preferentially expressed on some tumors such as medulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cell proliferation. CD140b has been shown to interact with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2, Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin β3 and nexin sorting molecules. CD140b has been implicated in several disease states including atherogenesis and oncogenesis. The PDGFRβ is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. 

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with human PDGFRbeta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 18A2 monclonal antibody recognizes human CD140b also known as the platelet-derived growth factor receptor, beta polypeptide, PDGFR1, and PDGFRß. It has been shown to be useful for flow cytometric detection of CD140b.

Application References

(PubMed link indicates BioLegend citation)
  1. Vogel W, et al. 2002. Haematologica 88:126.
  2. Arima, S., et al 2011. Development. 138:4763. PubMed.
Product Citations
  1. Costa A et al. 2018. Cancer cell. 33(3):463-479 . PubMed
  2. Sasayama S, et al. 2018. Int J Mol Sci. 19:. PubMed
  3. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  4. Krenzlin H, et al. 2019. J Clin Invest. 130:1671. PubMed
  5. Nguyen M et al. 2018. Cell reports. 25(13):3884-3893 . PubMed
  6. Lemcke H, et al. 2017. Sci Rep. 7.065972222. PubMed
  7. Remy M, et al. 2019. Cells. 1.364583333. PubMed
  8. Pelon F, et al. 2020. Nat Commun. 0.738888889. PubMed
  9. Vasilache A, et al. 2015. Brain Behav Immun. 48: 31-41. PubMed
RRID
AB_2299493 (BioLegend Cat. No. 323605)
AB_2268134 (BioLegend Cat. No. 323606)

Antigen Details

Structure
Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR1 and PD
Distribution

Widely expressed on a variety of mesenchymal-derived cells. Preferentially expressed in medulloblastoma.

Function
Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Has been implicated in atherogenesis and oncogenesis.
Interaction
Interacts with a number of kinases (including Raf-1, NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Grb2, Grb4, RasGAP, SHP2, SHC1, Crk, others). Has also been shown to associate with integrin β3 and nexin sorting m
Ligand/Receptor
Binds to B-chain containing PDGF molecules as well as protease-activated PDGF-C
Modification
Multiple tyrosine phosphorylation sites (Y549, Y581, Y716, Y740, Y751, Y763, Y771, Y775, Y857, Y1009, Y1021)
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Claesson-Welsh L, et al. 1988. Mol. Cell Biol. 8:3476.
2. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435.
3. Gilbertson DG, et al. 2001. J. Biol. Chem.276:27406.
4. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771.
5. Kanakaraj P, et al. 1991. Biochemistry 30:1761.

Gene ID
5159 View all products for this Gene ID
UniProt
View information about CD140b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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