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PE anti-human CD119 (IFN-γ R α chain) Antibody

Pricing & Availability
Clone
GIR-208 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-110
Other Names
IFN-γR, IFN-γRα, IFN-gamma R1, CDw119
Isotype
Mouse IgG1, κ
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Product Citations
publications
GIR-208_PE_CD119_Antibody_051018
Human peripheral blood lymphocytes stained with CD119 (clone GIR-208) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • GIR-208_PE_CD119_Antibody_051018
    Human peripheral blood lymphocytes stained with CD119 (clone GIR-208) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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308606 100 tests $244
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Description

CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IFN-γRα, Purified from human placenta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 under non-reducing conditions, immunoprecipitation1, immunohistochemistry3 of snap frozen sections, and blocking1 of IFN-? binding to IFN-? R a chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 308609 & 308610).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan K, et al. 1988. J. Immunol. 140:4231. (WB IP Block)
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Peyman JA, et al. 1992. J. Immunol. 149:2675. (IHC)
Product Citations
  1. McQueen BE, et al. 2020. Infect Immun. :88. PubMed
  2. Iwasa M, et al. 2019. Oncotarget. 10:1903. PubMed
  3. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
RRID
AB_314726 (BioLegend Cat. No. 308606)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution

Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts

Function
Activates Jak1, Jak2, STAT1, host defense
Ligand/Receptor
IFN-γ
Cell Type
B cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Calderon J, et al. 1988. P. Natl. Acad. Sci. USA 85:4837.
2. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
3. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.

Gene ID
3459 View all products for this Gene ID
UniProt
View information about CD119 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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