PE anti-human C5L2 Antibody

Pricing & Availability
Clone
1D9-M12 (See other available formats)
Regulatory Status
RUO
Other Names
GPR77, C5a receptor-like 2, C5a anaphylatoxin chemotactic receptor
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1D9-M12_PE_050409.jpg
Human peripheral blood granulocytes stained with 1D9-M12 PE
  • 1D9-M12_PE_050409.jpg
    Human peripheral blood granulocytes stained with 1D9-M12 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
342403 25 tests $150.00
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342404 100 tests $295.00
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Description

C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77, which shares significant homology to C5a receptor (CD88), but it is a decoy receptor without G-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such as neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 binds C5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than with C5aR.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: Western blotting1,3 and blocking2,3. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Clone 1D9-M12 is known to block C5a binding to C5L2, but does not react with C5a receptor. It has been reported that this clone is not effective in immunohistochemistry of formalin-fixed paraffin-embedded sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Fonseca MI, et al. 2013. J. Neuroinflammation. 10:25. (WB) PubMed
  2. Hao J, et al. 2013. PLoS One 8:e66305. (Block)
  3. Raby AC, et al. 2011. Eur. J. Immunol. 41:2741. (Block, WB)
Product Citations
  1. Schmidt T, et al. 2015. PLoS One. 10: 0132703. PubMed
  2. Spaan A, et al. 2015. J Immunol. 195: 1034-1043. PubMed
  3. Derer S, et al. 2015. J Immunol. 195: 5077 - 5087. PubMed
  4. Su S et al. 2018. Cell. 172(4):841-856 . PubMed
RRID
AB_2113253 (BioLegend Cat. No. 342403)
AB_2247831 (BioLegend Cat. No. 342404)

Antigen Details

Structure
Seven transmembrane protein, decoy receptor, does not couple to G-proteins.
Distribution

Neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes.

Function
Negative regulation of C5a function.
Ligand/Receptor
C5a, C5a-des-Arg.
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Scola AM, et al. 2009. Mol. Immunol. 46:1149
2. Johswich K, et al. 2006. J. Bio. Chem. 281:39088
3. Gerad NP, et al. 2005. J. Bio. Chem. 280:39677
4. Okinaga S, et al. 2003. Biochemistry. 42:9406

Gene ID
27202 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
C5L2
Specificity Alt (DOES NOT SHOW ON TDS):
C5L2
UniProt
View information about C5L2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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