PE anti-Cyclin D3 Antibody

Pricing & Availability
Clone
DCS-22 (See other available formats)
Regulatory Status
RUO
Other Names
G1/S-specific cyclin-D3, CCND3
Isotype
Mouse IgG1, κ
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Product Citations
publications
DCS-22_PE_CyclinD3_WB_ICFC_1_042417
PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and anti-Cyclin D3 (DCS-22) PE (top) or mouse IgG1, κ isotype control PE (bottom).
  • DCS-22_PE_CyclinD3_WB_ICFC_1_042417
    PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and anti-Cyclin D3 (DCS-22) PE (top) or mouse IgG1, κ isotype control PE (bottom).
  • DCS-22_PE_CyclinD3_WB_ICFC_2_042417
See PE spectral data See supplemental Ethanol data...
Cat # Size Price Quantity Check Availability Save
684903 25 tests $130.00
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684904 100 tests $280.00
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Description

D-type cyclins, including Cyclin D1, D2, and D3, are regulators of the Cyclin-dependent kinases 4 and 6 (Cdk4 and Cdk6) and mediate the growth factor-induced progression through the G1 phase in the cell cycle. Cyclins D1 and D3 are involved in cell-cycle progression in CDK-dependent manner and CDK-independent activation of transcription as well as metabolic control. CDKs promote cell cycle transitions in mammalian cells by phosphorylating key substrates cyclins. The active complex of cyclin D/CDK4 targets the retinoblastoma protein, which triggers the release of E2F transcription factors and activates G1/S-phase gene expression.

Abnormal expression of cyclins is involved in disease progression. Cyclin D3 is widely expressed in many tumor cells. Cyclin D3 gene is amplified in bladder carcinoma in situ. Genomic changes disrupting the expression of Cyclin D3 are associated with aberrant growth of several human B-lymphoid malignancies. Targeting Cyclin D3 by miR-138 could induce cell cycle arrest in hepatocellular carcinoma. Cyclin D3 was upregulated in breast cancer and could serve as predictor of breast cancer prognosis. Cyclin D3 can also be targeted in autoimmune diabetes and exerts a protective role on β cells by promoting their survival and fitness without affecting proliferation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length Cyclin D3 of human origin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2686979 (BioLegend Cat. No. 684903)
AB_2686980 (BioLegend Cat. No. 684904)

Antigen Details

Structure
Major isoform has 291 amino acids and predicted molecular weight of 33 kD.
Distribution

Nucleus, cytoplasm and cell membrane.

Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition.
Interaction
CDK14, CDK4, CDK6, ATF5, EIF3K, CDKN1A, ICP0, POLR3GL, and TFIP11.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References

1. Liu W, et al. 2004. Biochem. Biophys. Res. Commun. 321:954.
2. Sherr CJ. 1993. Cell 73:1059.
3. Saavedra-Ávila NA. 2014. Proc. Natl. Acad. Sci. USA 111:E3405.
4. Lukas J, et al. 1996. Mol. Cell Biol. 16:6917.
5. Wang W, et al. 2012. Carcinogenesis. 33:1113.
6. Cato MH, et al. 2011. Mol. Cell Biol. 31:127.
7. Zhang Q, et al. 2014. Mol. Cell Endocrinol. 382:583.
8. Chi Y, et al. 2015. Cancer Cell Int. 15:89.

Gene ID
896 View all products for this Gene ID
UniProt
View information about Cyclin D3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 04/24/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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