PE anti-Cyclin A Antibody

Pricing & Availability
Clone
E23.1 (See other available formats)
Regulatory Status
RUO
Other Names
CCNA2, cyclin A2, CCN1, CCNA
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
E23dot1_PE_Cyclin-A_Antibody_ICFC_1_081117
NIH/3T3 cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and anti-Cyclin A (clone E23.1) PE (top) or mouse IgG2a, κ isotype control PE (bottom).
  • E23dot1_PE_Cyclin-A_Antibody_ICFC_1_081117
    NIH/3T3 cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and anti-Cyclin A (clone E23.1) PE (top) or mouse IgG2a, κ isotype control PE (bottom).
  • E23dot1_PE_Cyclin-A_Antibody_ICFC_2_081117
  • E23dot1_PE_Cyclin-A_Antibody_ICFC_3_081417
    Jurkat cells were fixed and permiabilized with cold 70% ethanol then intracellularly stained with DAPI and anti-Cyclin A (clone E23.1) PE (top) or mouse IgG2a, κ isotype control PE (bottom).
  • E23dot1_PE_Cyclin-A_Antibody_ICFC_4_081417
See PE spectral data
Cat # Size Price Quantity Avail. Save
644003 25 tests $125
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644004 100 tests $275
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Description

Cyclins regulate the activity of the Cyclin-dependent protein kinases. Cyclin A is involved in the regulation of the cell cycle and seems to be essiential for S phase and M phase. Studies have shown that overexpression of cyclin A can accelerate the G1 to S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Bovine
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cyclin A fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Antibody may perform better for ICFC if cells sit in 70% ethanol or True Phos™ perm Buffer for 24 hours – 72 hours at -20° C

RRID
AB_2715836 (BioLegend Cat. No. 644003)
AB_2715837 (BioLegend Cat. No. 644004)

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Pagano M et al. 1992. EMBO J 11:961
2. Geley S et al. 2001 J Cell Biol. 153:137
3. Resinitzky D et al. 1995. Mol Cell Biol 15:4347
4. Furuno N et al. 1999. J Cell Biol. 147:295

Gene ID
890 View all products for this Gene ID
UniProt
View information about Cyclin A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 08/15/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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