Pan-Cytokeratin (AE1/AE3) Monoclonal Antibody, Purified

Pricing & Availability
AE-1/AE-3 (See other available formats)
Mouse IgG1, κ
Cat # Size Price Quantity Avail. Save
914202 6 mL $101
Check Availability

Need larger quantities of this item?
Request Bulk Quote

This product is currently unavailable.

914201 1 mL $201
Check Availability

Need larger quantities of this item?
Request Bulk Quote
This product is scheduled for discontinuation. Please use the availability checker to determine current availability.

This antibody is effective in immunohistochemistry (IHC).

The pan-cytokeratin AE1/AE3 antibody cocktail provides a broad spectrum of reactivity and stains low molecular weight and high molecular weight cytokeratins in virtually all epithelia.

AE1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.

Concentrated Format:
Cat. No. 914201 (Formerly: SIG-3464-1000) [1 mL]

Prediluted Formats:
Cat. No. 914202 (Formerly: SIG-3464-26) [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Product Details
Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody AE-1/AE-3 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Pan-Cytokeratin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

This antibody was developed using human epidermal keratins.
Phosphate-buffered solution with BSA + 0.1% NaN3.
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501)

Positive tissue (human): Skin

Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature

Product Citations
  1. Guernsey MW et al. 2017. eLife. 6 pii: e27450. PubMed
  2. Guernsey MW, et al. 2020. Mol Biol Evol. 37:2679. PubMed
AB_2565153 (BioLegend Cat. No. 914202)
AB_2565152 (BioLegend Cat. No. 914201)


  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 08/21/2020

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Remember me
Forgot your password? Reset Password
Request an Account