Pacific Blue™ anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RA3-6B2_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
  • RA3-6B2_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ RA3-6B2
See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
103230 25 µg $85.00
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103227 100 µg $195.00
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed
  2. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  3. Ilsley M, et al. 2017. Nucleic Acids Research. 10.1093/nar/gkx441. PubMed
  4. Hamilton J,et al. 2017. J Immunol. 10.4049/jimmunol.1700888. PubMed
  5. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  6. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  7. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  8. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  9. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  10. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  11. Courtney AH et al. 2017. Molecular cell. 67(3):498-511 . PubMed
  12. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  13. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  14. Tanaka Y, et al. 2017. J Immunol. 199:4016. PubMed
  15. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  16. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  17. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  18. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  19. Makdasi E, et al. 2009. J Immunol. 182:6143. PubMed
  20. Kat I, et al. 2010. Int Immunol. 0.986805556. PubMed
  21. Nishimori J, et al. 2012. Infect Immun . 80:4398. PubMed
  22. Eilat E 2013. J Immunol. 190:1472. PubMed
  23. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  24. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  25. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  26. Simerska P, et al. 2014. Vaccine. 32:4743. PubMed
  27. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  28. Günal-Sadık G, et al. 2014. PLoS One. 9:111748. PubMed
  29. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  30. Schmid C, et al. 2015. J Exp Med. 212:775. PubMed
  31. Vazquez S, et al. 2015. Exp Hematol. 43: 578-585. PubMed
  32. Grosjean F, et al. 2015. PLoS One. 10: 0133917. PubMed
  33. Kato Y, et al. 2015. J Immunol. 195: 1006-1014. PubMed
  34. Adachi Y, et al. 2015. J Exp Med. 212: 1709-1723. PubMed
  35. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  36. Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed
  37. Yoshikawa S, et al. 2016. Sci Rep. 6:18738. PubMed
  38. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  39. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  40. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  41. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  42. Onodera T, et al. 2016. J Immunol. 196: 4172 - 4184. PubMed
  43. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  44. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  45. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  46. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  47. Jyh Liang Hor, William R. Heath, Scott N. Mueller 2017. Sci Rep. 7:41091. PubMed
  48. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  49. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  50. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  51. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  52. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  53. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  54. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  55. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  56. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  57. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  58. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  59. Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed
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  61. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
RRID
AB_492877 (BioLegend Cat. No. 103230)
AB_492876 (BioLegend Cat. No. 103227)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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