- MEL-14 (See other available formats)
- Regulatory Status
- Other Names
- L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.Product Details
- Antibody Type
- Host Species
- C3H/eb mouse B lymphoma 38C-13
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).
(PubMed link indicates BioLegend citation)
- Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
- Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
- Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
- Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
- Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
- Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
- Olver S, et al. 2006. Cancer Res. 66:571.
- Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
- Lee JW, et al. 2006. Nature Immunol. 8:181.
- Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
- de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
- Product Citations
AB_493381 (BioLegend Cat. No. 104423)
AB_493380 (BioLegend Cat. No. 104424)
- Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
- Lymphocyte homing to HEV, rolling on activated endothelium
- CD34, GlyCAM-1, MAdCAM-1
- Cell Type
- B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.
- Gene ID
- 20343 View all products for this Gene ID
- View information about CD62L on UniProt.org
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