ELISA MAX™ Deluxe Set Mouse IgE

Pricing & Availability
Regulatory Status
RUO
Other Names
Mouse IgE ELISA Kit
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Product Citations
publications
mouse IgE_122109
  • mouse IgE_122109
Cat # Size Price Quantity Check Availability Save
432404 5 plates $260.00
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Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number, but will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum, Plasma and cell culture supernatant Immunoglobulin E (IgE) Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum, plasma and cell cultures.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IgE ELISA MAX™ Capture Antibody (200X)
  • Mouse IgE ELISA MAX™ Detection Antibody (200X)
  • Mouse IgE Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Wang W, et al. 2007. J. Immunol. 178:4885.
  2. Emara M, et al. 2011. J. Biol Chem. 286:13033. PubMed
  3. Hirose M, et al. 2011. J. Immunol. 187:1176. PubMed
  4. Ramon HE, et al. 2012. J. Immunol. 188:4023.PubMed
  5. Sawant DV, et al. 2014. J. Immunol. 192:2904. PubMed
  6. Ueha R, et al. 2014. Int Immunopharmacol. 22:242. PubMed
  7. Fritz JM, et al. 2014. PLoS One. 9:107473. PubMed
Product Citations
  1. Park HA, et al. 2019. Int J Mol Med. 43:1830. PubMed
  2. Hirose M, et al. 2011. J Immunol. 187:1176. PubMed
  3. Emara M, et al. 2011. J Biol Chem. 286:13033. PubMed
  4. Ramon H, et al. 2012. J Immunol. 188:4023. PubMed
  5. Sawant D, et al. 2014. J Immunol. 192:2904. PubMed
  6. Ueha R, et al. 2014. Int Immunopharmacol. 22:242. PubMed
  7. Fritz J, Weaver T 2014. PLoS One. 9:107473. PubMed
  8. Rizzo J, et al. 2016. Cell Death Differ. 10.1038/cdd.2015.162. PubMed
  9. Liu X, et al. 2020. J Invest Dermatol. 140:1856. PubMed
Sensitivity
0.1 ng/mL
Standard Range
0.156-10 ng/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 04/16/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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