ELISA MAX™ Deluxe Set Human CCL28

Pricing & Availability
Regulatory Status
RUO
Other Names
Mucosae-associated epithelial chemokine, MEC, CCK1, SCYA28, chemokine (C-C motif) ligand 28
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Product Citations
publications
CCL28_Human_ELISA_Kit_Deluxe_123113
  • CCL28_Human_ELISA_Kit_Deluxe_123113
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441204 5 plates $347
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA MAX™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA MAX™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

The ELISA MAX™ Deluxe Set is specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

CCL28 is also known as mucosa-associated epithelial chemokine (MEC) and CCK1. It is expressed by columnar epithelial cells in the gut, lung, breast and salivary glands, and its expression can be increased by a number of stimulants incluiding proinflammatory cytokines. It is involved in homing of T and B lymphocytes that express CCR10 and migration of eosinophils that express CCR3. CCL28 has been implicated in a number of disease processes including atopic dermatitis, asthma, psoriasis, vulgaris, and bullous pemphigoid.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human CCL28 ELISA MAX™ Capture Antibody (200X)
  • Human CCL28 ELISA MAX™ Detection Antibody (200X)
  • Human CCL28 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
8.0 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
56477 View all products for this Gene ID
UniProt
View information about CCL28 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 09/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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