HRP anti-SNAP-25 Antibody

Pricing & Availability
Clone
SP12 (See other available formats)
Regulatory Status
RUO
Other Names
Synaptosomal-associated protein 25, SUP, RIC-4, RIC4, SEC9, SNAP
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SP12_HRP_SNAP-25_Antibody_1_122319.png
Western blot of HRP anti-SNAP-25 antibody (clone SP12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • SP12_HRP_SNAP-25_Antibody_1_122319.png
    Western blot of HRP anti-SNAP-25 antibody (clone SP12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system.
  • SP12_HRP_SNAP-25_Antibody_2_122319.png
    IHC staining of HRP anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP12_HRP_SNAP-25_Antibody_3_122319.png
    IHC staining of HRP anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP12_HRP_SNAP-25_Antibody_4_122319.png
    IHC staining of HRP anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP12_HRP_SNAP-25_Antibody_5_122319.png
    IHC staining of HRP anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP12_HRP_SNAP-25_Antibody_6_122319.png
    IHC staining of HRP anti-SNAP-25 antibody (clone SP12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
805003 25 µg $125
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805004 100 µg $265
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Description

SNAP-25, also known as synaptosomal-associated protein 25, is a component of the trans-SNARE complex. It is a Q-SNARE protein anchored to the membrane on the cytosolic face, and does not contain a trans-membrane domain. The SNARE complex is involved in the vesicle fusion process. SNAP-25 is implicated in the inhibition of voltage-gated calcium channels, and has been shown to decrease calcium responsiveness in glutamatergic synapses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation from the post mortem human brain.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.001 - 0.02 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 2.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Honer WG, et al. 1993. Brain Res. 609:9-20.
  2. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
RRID
AB_2820123 (BioLegend Cat. No. 805003)
AB_2820124 (BioLegend Cat. No. 805004)

Antigen Details

Structure
Expected MW: 28 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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