HRP anti-rabbit IgG Antibody

Pricing & Availability
Clone
6B9G9 (See other available formats)
Regulatory Status
RUO
Other Names
N0034
Isotype
Mouse IgG1, κ
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Product Citations
publications
6B9G9_HRP_IgG_WB_Antibody_020717
Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with anti-β-actin antibody (clone Poly6221, rabbit IgG) (lane 1, 2, 3 and 5) and purified anti-β actin antibody as the negative control (clone 2F1-1, mouse IgG) (lane 4). After a 30 minutes wash, lanes 1 and 4, 2, 3 were probed respectively with 1:5000, 1:10000 and 1:20000 HRP anti-rabbit IgG antibodies (clone 6B9G9), and lane 5 was probed with 1:3000 HRP Donkey anti-rabbit IgG (minimal x-reactivity) antibody (clone Poly4064) as a positive control. Proteins were visualized using chemiluminescence detection system. Lane M contains the molecular weight ladder.
  • 6B9G9_HRP_IgG_WB_Antibody_020717
    Total cell lysate from HeLa (15 µg/lane) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with anti-β-actin antibody (clone Poly6221, rabbit IgG) (lane 1, 2, 3 and 5) and purified anti-β actin antibody as the negative control (clone 2F1-1, mouse IgG) (lane 4). After a 30 minutes wash, lanes 1 and 4, 2, 3 were probed respectively with 1:5000, 1:10000 and 1:20000 HRP anti-rabbit IgG antibodies (clone 6B9G9), and lane 5 was probed with 1:3000 HRP Donkey anti-rabbit IgG (minimal x-reactivity) antibody (clone Poly4064) as a positive control. Proteins were visualized using chemiluminescence detection system. Lane M contains the molecular weight ladder.
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410406 100 µL $275.00
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Technical data sheet

Product Details

Reactivity
Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rabbit IgG
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Optimal concentration of usage is 1:10,000 – 1:40,000 for WB. The optimal concentration should be determined by titration for each individual assay of interest.

 

Product Citations
  1. Wu YH, et al. 2020. Mol Med Rep. 21:851. PubMed
  2. Uka R, et al. 2020. Oncogene. 39:4132. PubMed
RRID
AB_2650737 (BioLegend Cat. No. 410406)

Antigen Details

Structure
IgG antibodies are large molecules of about 150 kD made of four peptide chains. It contains two identical class γ heavy chains of about 50 kD and two identical light chains of about 25 kD.
Distribution

Secreted, B cell surface.

Function
Antibodies are major components of humoral immunity. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. By binding many kinds of pathogens such as viruses, bacteria, and fungi, IgG protects the body from infection.
Gene ID
NA
UniProt
View information about IgG on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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