Go-ChIP-Grade™ Purified Rabbit Polyclonal Isotype Ctrl Antibody

Pricing & Availability
Clone
Poly29108 (See other available formats)
Regulatory Status
RUO
Other Names
Rabbit Polyclonal Antibody, Rabbit Polyclonal antibodies
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly29108_GoChIP_PURE_Rabbit_Poly_Iso-ctrl_062718
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone Poly6074, Cat. No. 607401) or B) equal amount of Purified Rabbit Polyclonal isotype ctrl antibody (Clone Poly29108, Cat. No. 910801) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • Poly29108_GoChIP_PURE_Rabbit_Poly_Iso-ctrl_062718
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone Poly6074, Cat. No. 607401) or B) equal amount of Purified Rabbit Polyclonal isotype ctrl antibody (Clone Poly29108, Cat. No. 910801) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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910805 25 µg $45.00
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910806 100 µg $100.00
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability.
Description

This antibody can be used as an isotype-matched control for several applications such as Chromatin Immunoprecipitation, Western Blot, ELISA and other immunostaining techniques.

Product Details
Technical data sheet

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was derived from rabbits.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, IHC-P, ELISA - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP assay as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

This antibody was subsequently purified using Protein G. The antibody enables an estimation of non-specific binding of rabbit polyclonal antibodies to cell surface components. This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Baghdadi M, et al. 2016. Cancer. Res. 76:6030-6042. PubMed

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Go To Top Version: 1    Revision Date: 06/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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