Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype Ctrl Antibody

Pricing & Availability
Clone
MPC-11 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
MPC-11_ChIP_IgG2b-k_ctrl_1_060618_updated
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MPC-11_ChIP_IgG2b-k_ctrl_1_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MPC-11_ChIP_IgG2b-k_ctrl_2_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400375) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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400375 25 µg $20.00
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400376 100 µg $70.00
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This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 400301 and 400302 are available as an alternative.
Description

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ChIP assay as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed
Product Citations
  1. Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/23/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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