GMP Ultra-LEAF™ Purified anti-GDNF Antibody

Pricing & Availability
Clone
A16099G (See other available formats)
Other Names
Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF), ATF1, ATF2, HSCR3, HFB1-GDNF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16099G_ULEAF_GDNF_GMP-ULEAF_070220.png
Recombinant human GFRα1 (black circles) binds to immobilized recombinant human GDNF (Cat. No. 760402). GMP anti-GDNF antibody (purple squares, clone A16099G) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody blocks the binding of 0.2 µg/mL recombinant human GFRα1 protein to 0.5 μg/mL of immobilized recombinant human GDNF with an ND50 range of 0.05 - 0.4 µg/mL
  • A16099G_ULEAF_GDNF_GMP-ULEAF_070220.png
    Recombinant human GFRα1 (black circles) binds to immobilized recombinant human GDNF (Cat. No. 760402). GMP anti-GDNF antibody (purple squares, clone A16099G) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody blocks the binding of 0.2 µg/mL recombinant human GFRα1 protein to 0.5 μg/mL of immobilized recombinant human GDNF with an ND50 range of 0.05 - 0.4 µg/mL
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670905 100 µg $495.00
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Description

Glial cell-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor RET receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, which include depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease because it can protect dopaminergic neurons from neurotoxins and promote neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and kidney development. Upregulated expression of GDNF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human GDNF
Formulation
0.1 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between recombinant human GFRα and recombinant human GDNF (Cat. No. 760402). The ND50 range is 0.05 - 0.4 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894578 (BioLegend Cat. No. 670905)
Disclaimer

GMP Ultra-LEAF™ antibodies. BioLegend GMP Ultra-LEAF™ antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency    
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of releaed products

BioLegend GMP Ultra-LEAF™ antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

GDNF is a secreted protein that is widely expressed in the developing and adult central nervous system.

Function
GDNF is a neurotrophic factor that promotes the development, differentiation, and the survival of dopaminergic neurons. It also participates in the recovery process after brain surgery.
Interaction
GDNF targets the neuronal cells expressing GDNF family receptors.
Ligand/Receptor
GDNF interacts with GDNF family receptor α-1 (GFRα1) and has a lower affinity with GFRα2, GFRα3, and GFRα4.
Cell Type
Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons, Schwann Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors
Antigen References
  1. Allen SJ, et al. 2013. Pharmacol Ther. 138:155.
  2. Hendrich J, et al. 2012. Neuroscience. 219:204.
  3. Hidalgo-Figueroa M, et al. 2012. J Neurosci. 32:864.
  4. Pascual A, et al. 2011. J Mol Endocrinol. 3:R83-92.
  5. Carnicella S, et al. 2009. Pharmacol Ther. 122:9.
  6. Sariola H, et al. 2003. J Cell Sci. 116:3855.
Gene ID
2668 View all products for this Gene ID
UniProt
View information about GDNF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/25/2020

For research and ex vivo cell processing use. Not for diagnostic or therapeutic procedures. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to reverse engineer functionally similar materials without written approval of BioLegend. By use of these products you accept the terms and conditions of any and all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research and ex vivo cell processing use only and not intended for human or animal diagnostic or therapeutic use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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