GMP Recombinant Human IL-10 (carrier-free)

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Other Names
Interleukin-10, B-TCGF, CSIF, TGIF
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GMP recombinant human IL-10 inhibits IFN-γ induction in PHA activated human PBMC in a dose-dependent manner with ED50 range of 0.025 - 0.25 ng/mL.
  • Human_IL-10_CF_GMP-RECOM_071321.png
    GMP recombinant human IL-10 inhibits IFN-γ induction in PHA activated human PBMC in a dose-dependent manner with ED50 range of 0.025 - 0.25 ng/mL.
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571014 25 µg $475.00
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571016 100 µg $1600.00
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Description

IL-10 was first described as a cytokine that is produced by T helper 2 (Th2) cell clones. It inhibits interferon (IFN)-γ synthesis in Th1 cells, and therefore it was initially called cytokine synthesis inhibiting factor (CSIF).  Macrophages are the main source of IL-10 and its secretion can be stimulated by endotoxin (via Toll-like receptor 4, NF-κB dependent), tumor necrosis factor TNF-α (via TNF receptor p55, NF-κB-dependent), catecholamines, and IL-1. IL-10 controls inflammatory processes by suppressing the expression of proinflammatory cytokines, chemokines, adhesion molecules, as well as antigen-presenting and costimulatory molecules in monocytes/macrophages, neutrophils, and T cells. IL-10 inhibits the production of proinflammatory mediators by monocytes and macrophages such as endotoxin- and IFN-γ-induced release of IL-1α, IL-6, IL-8, G-CSF, GM-CSF, and TNF-α. In addition, it enhances the production of anti-inflammatory mediators such as IL-1RA and soluble TNFα receptors. IL-10 inhibits the capacity of monocytes and macrophages to present antigen to T cells. This is realized by down-regulation of constitutive and IFN-γ-induced cell surface levels of MHC class II, of costimulatory molecules such as CD86 and of some adhesion molecules such as CD58.

Product Details
Technical data sheet

Product Details

Source
Human IL-10, amino acids Ser19-Asn178 (Accession # NM_000572), was expressed in E.coli.
Molecular Mass
The 160 amino acid recombinant protein has a predicted molecular mass of 18 kD. The DTT-reduced protein migrates at approximately 18 kD and the non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The N-terminal amino acid is Serine.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.1 µm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.025 - 0.25 ng/mL as determined by the dose-dependent inhibition of IFN-γ induction in PHA activated human PBMC.
Application

Bioassay
Cell Culture

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency   
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of releaed products

BioLegend GMP recombinant protiens are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Cytokine
Distribution

IL-10 is produce by Th2 cells, macrophages, DCs, B cells, CD8+ T cells, regulatory T cells (Tregs), Th1 cells and Th17 cells. In addition, IL-10 is expressed by monocytes, B cells, eosinophils, and mast cells.

Function
IL-10 is an immunoregulatory cytokine. Its main function is the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of several immune cells such as T cells, B cells, natural killer cells, antigen-presenting cells, mast cells, and granulocytes.
Interaction
IL-10R is expressed in monocytes, NK, B and T cells. In addition, Langerhans cells, dermal dendritic cells, eosinophils, mast cells, and endothelial cells can respond to IL-10.
Ligand/Receptor
IL-10R1 and IL-10R2
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fiorentino DF, et al. 1989. J Exp Med. 170:2081-2095.
  2. Ho AS, et al. 1993. P. Natl. Acad. Sci. USA. 90:11267-11271.
  3. Hart PH, et al. 1996. J Immunol. 157:3672-3680.
  4. Asadullah K, et al. 2003. Pharmacol Rev. 55:241-269.
  5. Mosser DM and Zhang X. 2008. Immunol Rev. 226:205-218.
  6. Maynard CL and Weaver CT. 2008. Immunol Rev. 226:219-233.
Gene ID
3586 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/22/2021

For research and ex vivo cell processing use. Not for diagnostic or therapeutic procedures. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to reverse engineer functionally similar materials without written approval of BioLegend. By use of these products you accept the terms and conditions of any and all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research and ex vivo cell processing use only and not intended for human or animal diagnostic or therapeutic use.

 

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